› 3mg1

X-ray diffraction
1.65Å resolution

Crystal structure of the orange carotenoid protein from cyanobacteria Synechocystis sp. PCC 6803

Released:
DOI: 10.2210/pdb3mg1/pdb
PDB entry 3mg1 coloured by chain, front view.
PDB entry 3mg1 coloured by chain, side view.
PDB entry 3mg1 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Structural determinants underlying photoprotection in the photoactive orange carotenoid protein of cyanobacteria.
Wilson A, Kinney JN, Zwart PH, Punginelli C, D'Haene S, Perreau F, Klein MG, Kirilovsky D, Kerfeld CA
J Biol Chem 285 18364-75 (2010)
PMID: 20368334

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orange carotenoid-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 35.46 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P74102 (Residues: 1-316; Coverage: 100%)
Gene name: slr1963
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand ECH 2 x ECH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P32
Unit cell:
a: 82.939Å b: 82.939Å c: 87.632Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.162 0.19
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Conditionally and transiently disordered proteins: awakening cryptic disorder to regulate protein function.
Jakob et al. (2014)
10 more

27 mentions without citation

Recent Progress in Solution Structure Studies of Photosynthetic Proteins Using Small-Angle Scattering Methods.
Golub et al. (2023)
26 more