› 3mfs

X-ray diffraction
2.1Å resolution

CASK-4M CaM Kinase Domain, AMPPNP

Released:
DOI: 10.2210/pdb3mfs/pdb
PDB entry 3mfs coloured by chain, front view.
PDB entry 3mfs coloured by chain, side view.
PDB entry 3mfs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Evolution of CASK into a Mg2+-sensitive kinase.
Mukherjee K, Sharma M, Jahn R, Wahl MC, Südhof TC
Sci Signal 3 ra33 (2010)
PMID: 20424264

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peripheral plasma membrane protein CASK Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14936 (Residues: 1-337; Coverage: 36%)
Gene names: CASK, LIN2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 59.006Å b: 62.157Å c: 97.899Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.268
Expression system: Escherichia coli

Quick links

Citations

10 review citations

CaM Kinase: Still Inspiring at 40.
Bayer et al. (2019)
9 more

3 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
2 more