3mfe

X-ray diffraction
2.6Å resolution

Crystal Structure of Mycobacterium Tuberculosis Proteasome open-gate mutant with H0 movement

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit beta Chains: 2, C, E, H, J, L, N, P, R, T, X, Z
Molecule details ›
Chains: 2, C, E, H, J, L, N, P, R, T, X, Z
Length: 240 amino acids
Theoretical weight: 25.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: G, V
Molecule details ›
Chains: G, V
Length: 240 amino acids
Theoretical weight: 25.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Length: 240 amino acids
Theoretical weight: 25.97 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 10-248; Coverage: 96%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 118.968Å b: 207.554Å c: 142.275Å
α: 90° β: 102.42° γ: 90°
R-values:
R R work R free
0.224 0.224 0.257
Expression system: Escherichia coli