› 3met

X-ray diffraction
2Å resolution

Crystal structure of SGF29 in complex with H3K4me2

Released:
DOI: 10.2210/pdb3met/pdb
PDB 3met coloured by chain and viewed from the front.
PDB 3met coloured by chain and viewed from the side.
PDB 3met coloured by chain and viewed from the top.
Source organisms:
Entry authors: Bian CB, Xu C, Lam R, Bountra C, Arrowsmith CH, Weigelt J, Edwards AM, Bochkarev A, Min J, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SAGA-associated factor 29 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96ES7 (Residues: 115-293; Coverage: 61%)
Gene names: CCDC101, SGF29
Sequence domains: SGF29 tudor-like domain
Structure domains: SH3 type barrels.
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Xenopus laevis
Expression system: Not provided
UniProt:
  • Canonical: Q92133 (Residues: 2-12; Coverage: 8%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c

Ligands and Environments

3 bound ligands:
Ligand UNX 3 x UNX
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
2 modified residues:
Ligand MSE 4 x MSE
Ligand MLY 2 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 50.162Å b: 65.411Å c: 105.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.243
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

44 review citations

"Reading" a new chapter in protozoan parasite transcriptional regulation.
Fleck et al. (2021)
43 more

8 mentions without citation

Identification of a GABAA receptor anesthetic binding site at subunit interfaces by photolabeling with an etomidate analog.
Li et al. (2006)
7 more