3met

X-ray diffraction
2Å resolution

Crystal structure of SGF29 in complex with H3K4me2

Released:
Source organisms:
Entry authors: Bian CB, Xu C, Lam R, Bountra C, Arrowsmith CH, Weigelt J, Edwards AM, Bochkarev A, Min J, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SAGA-associated factor 29 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96ES7 (Residues: 115-293; Coverage: 61%)
Gene names: CCDC101, SGF29
Sequence domains: SGF29 tudor-like domain
Structure domains: SH3 type barrels.
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.28 KDa
Source organism: Xenopus laevis
Expression system: Not provided
UniProt:
  • Canonical: Q92133 (Residues: 2-12; Coverage: 8%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c

Ligands and Environments

3 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 50.162Å b: 65.411Å c: 105.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.243
Expression systems:
  • Escherichia coli BL21
  • Not provided