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Crystal Structure of Staphylococcal Enterotoxin G (SEG) in Complex with a Mouse T-cell Receptor beta Chain

X-ray diffraction
2Å resolution
PDB entry 3mc0 coloured by chain, front view.
PDB entry 3mc0 coloured by chain, side view.
PDB entry 3mc0 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Enterotoxin SEG and TR-beta light chain
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Multimeric state: hetero dimer
Accessible surface area: 15512.53 Å2
Buried surface area: 2149.38 Å2
Dissociation area: 665.24 Å2
Dissociation energy (ΔGdiss): -4.3 kcal/mol
Dissociation entropy (TΔSdiss): 11.4 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106899
Assembly 2
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Assembly Name: Enterotoxin SEG and TR-beta light chain
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Multimeric state: hetero dimer
Accessible surface area: 15458.93 Å2
Buried surface area: 2087.24 Å2
Dissociation area: 652.13 Å2
Dissociation energy (ΔGdiss): -4.46 kcal/mol
Dissociation entropy (TΔSdiss): 11.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106899

Macromolecules

Ig-like domain-containing protein
Chains: A, C
Length: 109 amino acids
Theoretical weight: 11.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2NTY6 (Residues: 31-144; Coverage: 96%)
Pfam: Immunoglobulin V-set domain
InterPro:
CATH: Immunoglobulins
Ig-like domain-containing protein in PDB entry 3mc0, assembly 1, front view.
Ig-like domain-containing protein in PDB entry 3mc0, assembly 1, side view.
Ig-like domain-containing protein in PDB entry 3mc0, assembly 1, top view.
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Enterotoxin SEG
Chains: B, D
Length: 239 amino acids
Theoretical weight: 27.85 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D0EMB6 (Residues: 1-233; Coverage: 100%)
Pfam:
InterPro:
CATH:
Enterotoxin SEG in PDB entry 3mc0, assembly 1, front view.
Enterotoxin SEG in PDB entry 3mc0, assembly 1, side view.
Enterotoxin SEG in PDB entry 3mc0, assembly 1, top view.
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