3maa

X-ray diffraction
3Å resolution

Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Adenosine 5-O-(l-Thiophosphate) and Low Ca Concentration

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenylate cyclase type 5 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.53 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P30803 (Residues: 444-661; Coverage: 17%)
Gene name: ADCY5
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Adenylate cyclase type 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26769 (Residues: 870-1081; Coverage: 19%)
Gene name: Adcy2
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 394 amino acids
Theoretical weight: 45.77 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21212
Unit cell:
a: 118.744Å b: 133.722Å c: 70.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.309
Expression system: Escherichia coli