› 3m8u

X-ray diffraction
1.85Å resolution

Crystal structure of glutathione-binding protein A (GbpA) from Haemophilus parasuis SH0165 in complex with glutathione disulfide (GSSG)

Released:
DOI: 10.2210/pdb3m8u/pdb
PDB entry 3m8u coloured by chain, front view.
PDB entry 3m8u coloured by chain, side view.
PDB entry 3m8u coloured by chain, top view.
Source organism: Glaesserella parasuis SH0165
Primary publication:
Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.
Vergauwen B, Elegheert J, Dansercoer A, Devreese B, Savvides SN
Proc Natl Acad Sci U S A 107 13270-5 (2010)
PMID: 20628015

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 58.65 KDa
Source organism: Glaesserella parasuis SH0165
Expression system: Escherichia coli
UniProt:
  • Canonical: B8F653 (Residues: 24-531; Coverage: 100%)
Gene names: HAPS_1202, hbpA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDS 1 x GDS
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 57.52Å b: 68.47Å c: 142.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.188
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Recent insights into iron import by bacteria.
Braun et al. (2011)
8 more

8 mentions without citation

Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.
Vergauwen et al. (2010)
7 more