› 3m8u

X-ray diffraction
1.85Å resolution

Crystal structure of glutathione-binding protein A (GbpA) from Haemophilus parasuis SH0165 in complex with glutathione disulfide (GSSG)

Released:
DOI: 10.2210/pdb3m8u/pdb
PDB 3m8u coloured by chain and viewed from the front.
PDB 3m8u coloured by chain and viewed from the side.
PDB 3m8u coloured by chain and viewed from the top.
Source organism: Glaesserella parasuis SH0165
Primary publication:
Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.
Vergauwen B, Elegheert J, Dansercoer A, Devreese B, Savvides SN
Proc Natl Acad Sci U S A 107 13270-5 (2010)
PMID: 20628015

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SBP_bac_5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 522 amino acids
Theoretical weight: 58.65 KDa
Source organism: Glaesserella parasuis SH0165
Expression system: Escherichia coli
UniProt:
  • Canonical: B8F653 (Residues: 24-531; Coverage: 100%)
Gene names: HAPS_1202, hbpA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDS 1 x GDS
Ligand MLI 3 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 57.52Å b: 68.47Å c: 142.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.188
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Redox regulation by reversible protein S-thiolation in bacteria.
Loi et al. (2015)
8 more

5 mentions without citation

Glutathione import in Haemophilus influenzae Rd is primed by the periplasmic heme-binding protein HbpA.
Vergauwen et al. (2010)
4 more