› 3m38

X-ray diffraction
1.42Å resolution

The roles of Glutamates and Metal ions in a rationally designed nitric oxide reductase based on myoglobin: I107E FeBMb (No metal ion binding to FeB site)

Released:
DOI: 10.2210/pdb3m38/pdb
PDB entry 3m38 coloured by chain, front view.
PDB entry 3m38 coloured by chain, side view.
PDB entry 3m38 coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Roles of glutamates and metal ions in a rationally designed nitric oxide reductase based on myoglobin.
Lin YW, Yeung N, Gao YG, Miner KD, Tian S, Robinson H, Lu Y
Proc Natl Acad Sci U S A 107 8581-6 (2010)
PMID: 20421510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.3 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 39.85Å b: 46.98Å c: 77.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 not available not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Beyond directed evolution--semi-rational protein engineering and design.
Lutz S. (2010)
14 more

6 mentions without citation

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
5 more