› 3m34

X-ray diffraction
1.65Å resolution

Crystal structure of transketolase in complex with thiamin diphosphate and calcium ion

Released:
DOI: 10.2210/pdb3m34/pdb
PDB entry 3m34 coloured by chain, front view.
PDB entry 3m34 coloured by chain, side view.
PDB entry 3m34 coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Entry authors: Nocek B, Makowska-Grzyska M, Maltseva N, Grimshaw S, Joachimiak A, Anderson W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 635 amino acids
Theoretical weight: 70.42 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P7Y3 (Residues: 1-632; Coverage: 100%)
Gene names: Cj1645, tkt
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP
3 bound ligands:
Ligand GOL 2 x GOL
Ligand CA 1 x CA
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 130.35Å b: 70.819Å c: 69.236Å
α: 90° β: 109.92° γ: 90°
R-values:
R R work R free
0.17 0.151 0.182
Expression system: Escherichia coli BL21(DE3)

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