› 3m1l

X-ray diffraction
2.52Å resolution

Crystal structure of a C-terminal trunacted mutant of a putative ketoacyl reductase (FabG4) from Mycobacterium tuberculosis H37Rv at 2.5 Angstrom resolution

Released:
DOI: 10.2210/pdb3m1l/pdb
PDB 3m1l coloured by chain and viewed from the front.
PDB 3m1l coloured by chain and viewed from the side.
PDB 3m1l coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Crystal structure of FabG4 from Mycobacterium tuberculosis reveals the importance of C-terminal residues in ketoreductase activity.
Dutta D, Bhattacharyya S, Mukherjee S, Saha B, Das AK
J. Struct. Biol. 174 147-55 (2011)
PMID: 21081168

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-(Acyl-carrier-protein) reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 432 amino acids
Theoretical weight: 44.56 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O53665 (Residues: 17-448; Coverage: 95%)
Gene names: MT0256, fabG-1
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 65.076Å b: 80.497Å c: 153.953Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.241
Expression system: Escherichia coli

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Citations

9 citation in other articles

Crystal structure of hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis.
Dutta et al. (2013)
8 more