› 3m0l

X-ray diffraction
1.85Å resolution

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant S329F in complex with D-psicose

Released:
DOI: 10.2210/pdb3m0l/pdb
PDB entry 3m0l coloured by chain, front view.
PDB entry 3m0l coloured by chain, side view.
PDB entry 3m0l coloured by chain, top view.
Source organism: Pseudomonas stutzeri
Primary publication:
Elucidation of the role of Ser329 and the C-terminal region in the catalytic activity of Pseudomonas stutzeri L-rhamnose isomerase.
Yoshida H, Takeda K, Izumori K, Kamitori S
Protein Eng Des Sel 23 919-27 (2010)
PMID: 20977999

Function and Biology Details

Reaction catalysed: 
L-rhamnopyranose = L-rhamnulose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181127 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase-like TIM barrel domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 438 amino acids
Theoretical weight: 48.06 KDa
Source organism: Pseudomonas stutzeri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WH8 (Residues: 1-430; Coverage: 100%)
Gene name: L-RhI
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
Ligand MN 8 x MN
Ligand PSJ 4 x PSJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 74.576Å b: 104.117Å c: 111.23Å
α: 90° β: 106.21° γ: 90°
R-values:
R R work R free
0.181 0.178 0.21
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Regulation of the rhaEWRBMA Operon Involved in l-Rhamnose Catabolism through Two Transcriptional Factors, RhaR and CcpA, in Bacillus subtilis.
Hirooka et al. (2015)
1 more