PDBe 3lxr

X-ray diffraction
1.68Å resolution

Shigella IpgB2 in complex with human RhoA and GDP (complex C)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 2-181; Coverage: 93%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
IpgB2 Chain: F
Molecule details ›
Chain: F
Length: 192 amino acids
Theoretical weight: 21.94 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AJW7 (Residues: 1-188; Coverage: 100%)
Gene names: SAMEA3710404_01071, SFLP025, SF_p0024, ipgB2
Sequence domains: IpaB/EvcA family
Structure domains: SopE-like GEF fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 77.58Å b: 100.79Å c: 50.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.219
Expression system: Escherichia coli