› 3lx4

X-ray diffraction
1.97Å resolution

Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(deltaEFG)

Released:
DOI: 10.2210/pdb3lx4/pdb
PDB entry 3lx4 coloured by chain, front view.
PDB entry 3lx4 coloured by chain, side view.
PDB entry 3lx4 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(DeltaEFG).
Mulder DW, Boyd ES, Sarma R, Lange RK, Endrizzi JA, Broderick JB, Peters JW
Nature 465 248-51 (2010)
PMID: 20418861

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron hydrogenase small subunit domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 49.37 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FYU1 (Residues: 57-497; Coverage: 89%)
Gene names: CHLRE_03g199800v5, hyd1, hydA, hydA1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand CL 2 x CL
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P32
Unit cell:
a: 70.895Å b: 70.895Å c: 155.429Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.216
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
29 more

13 mentions without citation

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
12 more