PDBe 3lwn

X-ray diffraction
2.28Å resolution

Shigella IpgB2 in complex with human RhoA, GDP and Mg2+ (complex B)


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transforming protein RhoA Chains: A, B
Molecule details ›
Chains: A, B
Length: 185 amino acids
Theoretical weight: 20.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P61586 (Residues: 2-181; Coverage: 93%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
IpgB2 Chains: F, G
Molecule details ›
Chains: F, G
Length: 192 amino acids
Theoretical weight: 21.94 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
  • Canonical: Q9AJW7 (Residues: 1-188; Coverage: 100%)
Gene names: SAMEA3710404_01071, SFLP025, SF_p0024, ipgB2
Sequence domains: IpaB/EvcA family
Structure domains: SopE-like GEF fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 75.53Å b: 95.7Å c: 102.96Å
α: 90° β: 90° γ: 90°
R R work R free
0.209 0.204 0.291
Expression system: Escherichia coli