› 3lsa

X-ray diffraction
1.79Å resolution

Padron0.9-OFF (non-fluorescent state)

Released:
DOI: 10.2210/pdb3lsa/pdb
PDB entry 3lsa coloured by chain, front view.
PDB entry 3lsa coloured by chain, side view.
PDB entry 3lsa coloured by chain, top view.
Source organism: Pectiniidae
Primary publication:
Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron.
Brakemann T, Weber G, Andresen M, Groenhof G, Stiel AC, Trowitzsch S, Eggeling C, Grubmüller H, Hell SW, Wahl MC, Jakobs S
J Biol Chem 285 14603-9 (2010)
PMID: 20236929

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Padron0.9 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.97 KDa
Source organism: Pectiniidae
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

4 bound ligands:
Ligand SPD 3 x SPD
Ligand GOL 4 x GOL
Ligand SO4 1 x SO4
Ligand EPE 2 x EPE
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 73.334Å b: 104.262Å c: 123.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.19
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Fluorescent proteins for live-cell imaging with super-resolution.
Nienhaus et al. (2014)
11 more

3 mentions without citation

Molecular basis of the light-driven switching of the photochromic fluorescent protein Padron.
Brakemann et al. (2010)
2 more