3lm9

X-ray diffraction
2.45Å resolution

Crystal structure of fructokinase with ADP and Fructose bound in the active site

Released:

Function and Biology Details

Reaction catalysed:
ATP + D-fructose = ADP + D-fructose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative fructokinase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.08 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O05510 (Residues: 1-299; Coverage: 100%)
Gene names: BSU05860, gmuE, ydhR
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3121
Unit cell:
a: 112.333Å b: 112.333Å c: 73.958Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.196
Expression system: Escherichia coli BL21(DE3)