3lkk

X-ray diffraction
2Å resolution

Crystal structure of the isopentenyl phosphate kinase substrate complex

Released:
Primary publication:
X-ray structures of isopentenyl phosphate kinase.
ACS Chem. Biol. 5 517-27 (2010)
PMID: 20402538

Function and Biology Details

Reaction catalysed:
ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.54 KDa
Source organism: Thermoplasma acidophilum DSM 1728
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HLX1 (Residues: 1-245; Coverage: 100%)
Gene name: Ta0103
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 124.307Å b: 44.279Å c: 91.764Å
α: 90° β: 109.45° γ: 90°
R-values:
R R work R free
0.179 0.176 0.231
Expression system: Escherichia coli BL21(DE3)