› 3lh5

X-ray diffraction
2.6Å resolution

Crystal Structure of the SH3-Guanylate kinase core domain of ZO-1

Released:
DOI: 10.2210/pdb3lh5/pdb
PDB entry 3lh5 coloured by chain, front view.
PDB entry 3lh5 coloured by chain, side view.
PDB entry 3lh5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into regulated ligand binding sites from the structure of ZO-1 Src homology 3-guanylate kinase module.
Lye MF, Fanning AS, Su Y, Anderson JM, Lavie A
J Biol Chem 285 13907-17 (2010)
PMID: 20200156

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tight junction protein ZO-1 Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 29.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07157 (Residues: 516-803; Coverage: 14%)
Gene names: TJP1, ZO1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P64
Unit cell:
a: 125.8Å b: 125.8Å c: 35.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.205 0.285
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Tight junctions: from simple barriers to multifunctional molecular gates.
Zihni et al. (2016)
8 more

4 mentions without citation

The Src homology 3 domain is required for junctional adhesion molecule binding to the third PDZ domain of the scaffolding protein ZO-1.
Nomme et al. (2011)
3 more