› 3lb9

X-ray diffraction
3Å resolution

Crystal structure of the B. circulans cpA123 circular permutant

Released:
DOI: 10.2210/pdb3lb9/pdb
PDB entry 3lb9 coloured by chain, front view.
PDB entry 3lb9 coloured by chain, side view.
PDB entry 3lb9 coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Circular permutation of Bacillus circulans xylanase: a kinetic and structural study.
Reitinger S, Yu Y, Wicki J, Ludwiczek M, D'Angelo I, Baturin S, Okon M, Strynadka NC, Lutz S, Withers SG, McIntosh LP
Biochemistry 49 2464-74 (2010)
PMID: 20163191

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 182 amino acids
Theoretical weight: 19.99 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09850 (Residues: 23-27, 29-146; Coverage: 63%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 69.81Å b: 114.97Å c: 65.03Å
α: 90° β: 113.47° γ: 90°
R-values:
R R work R free
0.195 0.192 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Circular permutation: a different way to engineer enzyme structure and function.
Yu et al. (2011)
1 more