3laj

X-ray diffraction
2.31Å resolution

The Structure of the Intermediate Complex of the Arginine Repressor from Mycobacterium tuberculosis Bound to its DNA Operator and L-arginine.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Arginine repressor Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 170 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPY9 (Residues: 1-170; Coverage: 100%)
Gene names: MTCY06H11.22, Rv1657, ahrC, argR
Sequence domains:
Structure domains:
5'-D(*TP*TP*GP*CP*AP*TP*AP*AP*CP*GP*AP*TP*GP*CP*AP*A)-3' Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
5'-D(*TP*TP*GP*CP*AP*TP*CP*GP*TP*TP*AP*TP*GP*CP*AP*A)-3' Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 185.686Å b: 106.788Å c: 119.08Å
α: 90° β: 121.75° γ: 90°
R-values:
R R work R free
0.185 0.184 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided