3la8

X-ray diffraction
1.8Å resolution

The Crystal Structure of smu.1229 from Streptococcus mutans UA159

Released:
Source organism: Streptococcus mutans UA159
Entry authors: Su X-D, Hou QM, Liu X

Function and Biology Details

Reaction catalysed:
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 32.43 KDa
Source organism: Streptococcus mutans UA159
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DTU4 (Residues: 1-269; Coverage: 100%)
Gene names: SMU_1229, punA
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: R3
Unit cell:
a: 113Å b: 113Å c: 60.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.206 0.245
Expression system: Escherichia coli BL21(DE3)