› 3la6

X-ray diffraction
3.2Å resolution

Octameric kinase domain of the E. coli tyrosine kinase Wzc with bound ADP

Released:
DOI: 10.2210/pdb3la6/pdb
PDB entry 3la6 coloured by chain, front view.
PDB entry 3la6 coloured by chain, side view.
PDB entry 3la6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Identification of structural and molecular determinants of the tyrosine-kinase Wzc and implications in capsular polysaccharide export.
Bechet E, Gruszczyk J, Terreux R, Gueguen-Chaignon V, Vigouroux A, Obadia B, Cozzone AJ, Nessler S, Grangeasse C
Mol Microbiol 77 1315-25 (2010)
PMID: 20633230

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase wzc Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 286 amino acids
Theoretical weight: 31.68 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P76387 (Residues: 447-720; Coverage: 38%)
Gene names: JW2045, b2060, wzc
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 16 x ADP
Ligand CA 16 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 245.165Å b: 137.782Å c: 158.958Å
α: 90° β: 92.99° γ: 90°
R-values:
R R work R free
0.186 0.186 0.223
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial tyrosine kinases: evolution, biological function and structural insights.
Grangeasse et al. (2012)
4 more

12 mentions without citation

Targeting the Sugary Armor of Klebsiella Species.
Patro et al. (2019)
11 more