› 3l9x

X-ray diffraction
2.1Å resolution

KefC C-terminal domain in complex with KefF and ESG

Released:
DOI: 10.2210/pdb3l9x/pdb
PDB entry 3l9x coloured by chain, front view.
PDB entry 3l9x coloured by chain, side view.
PDB entry 3l9x coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Mechanism of ligand-gated potassium efflux in bacterial pathogens.
Roosild TP, Castronovo S, Healy J, Miller S, Pliotas C, Rasmussen T, Bartlett W, Conway SJ, Booth IR
Proc Natl Acad Sci U S A 107 19784-9 (2010)
PMID: 21041667

Function and Biology Details

Reaction catalysed: 
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione-regulated potassium-efflux system ancillary protein KefF; Glutathione-regulated potassium-efflux system protein KefC Chains: A, B
Molecule details ›
Chains: A, B
Length: 413 amino acids
Theoretical weight: 46.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03819 (Residues: 401-620; Coverage: 36%)
  • Canonical: P0A754 (Residues: 1-176; Coverage: 100%)
Gene names: JW0045, JW0046, b0046, b0047, kefC, kefF, trkC, yabF
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
4 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 2 x SO4
Ligand ESG 1 x ESG
Ligand L9X 1 x L9X
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 64.949Å b: 85.941Å c: 189.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Structure of potassium channels.
Kuang et al. (2015)
6 more

2 mentions without citation

The Potassium Efflux System Kef: Bacterial Protection against Toxic Electrophilic Compounds.
Rasmussen T. (2023)
1 more