› 3l3h

X-ray diffraction
2.7Å resolution

X-ray crystal structure of the F6A mutant of influenza A acid polymerase epitope PA224 bound to murine H2-Db MHC

Released:
DOI: 10.2210/pdb3l3h/pdb
PDB entry 3l3h coloured by chain, front view.
PDB entry 3l3h coloured by chain, side view.
PDB entry 3l3h coloured by chain, top view.
Source organisms:
Primary publication:
Constraints within major histocompatibility complex class I restricted peptides: presentation and consequences for T-cell recognition.
Theodossis A, Guillonneau C, Welland A, Ely LK, Clements CS, Williamson NA, Webb AI, Wilce JA, Mulder RJ, Dunstone MA, Doherty PC, McCluskey J, Purcell AW, Turner SJ, Rossjohn J
Proc Natl Acad Sci U S A 107 5534-9 (2010)
PMID: 20212169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135017 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 32.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 26-300; Coverage: 81%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Polymerase acidic protein Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.11 KDa
Source organism: Influenza A virus (A/African starling/England-Q/983/1979(H7N1))
Expression system: Not provided
UniProt:
  • Canonical: Q17TI0 (Residues: 224-233; Coverage: 1%)
Gene name: PA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 56.068Å b: 56.068Å c: 275.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
6 more

1 mention without citation

Constraints within major histocompatibility complex class I restricted peptides: presentation and consequences for T-cell recognition.
Theodossis et al. (2010)