› 3l0l

X-ray diffraction
1.74Å resolution

Crystal structure of orphan nuclear receptor RORgamma in complex with natural ligand

Released:
DOI: 10.2210/pdb3l0l/pdb
PDB entry 3l0l coloured by chain, front view.
PDB entry 3l0l coloured by chain, side view.
PDB entry 3l0l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for hydroxycholesterols as natural ligands of orphan nuclear receptor RORgamma.
Jin L, Martynowski D, Zheng S, Wada T, Xie W, Li Y
Mol Endocrinol 24 923-9 (2010)
PMID: 20203100

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear receptor ROR-gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 28.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51449 (Residues: 260-507; Coverage: 48%)
Gene names: NR1F3, RORC, RORG, RZRG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chains: C, E
Molecule details ›
Chains: C, E
Length: 13 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15596 (Residues: 685-697; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
Ligand HC3 2 x HC3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 66.775Å b: 86.262Å c: 91.945Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.252
Expression system: Escherichia coli

Quick links

Citations

39 review citations

25-Hydroxycholesterols in innate and adaptive immunity.
Cyster et al. (2014)
38 more

15 mentions without citation

ROR nuclear receptors: structures, related diseases, and drug discovery.
Zhang et al. (2015)
14 more