X-ray diffraction
2.7Å resolution

Crystal structure of cytosol aminopeptidase from Staphylococcus aureus COL

Entry authors: Hattne J, Dubrovska I, Halavaty A, Minasov G, Scott P, Shuvalova L, Winsor J, Otwinowski Z, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-101621 (preferred)
Entry contents:
1 distinct polypeptide molecule
Cytosol aminopeptidase domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 515 amino acids
Theoretical weight: 56.93 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
  • Canonical: A0A0H2WYH0 (Residues: 1-491; Coverage: 100%)
Gene name: SACOL0945
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 143.383Å b: 154.733Å c: 340.101Å
α: 90° β: 90° γ: 90°
R R work R free
0.222 0.221 0.24
Expression system: Escherichia coli