› 3kxi

X-ray diffraction
2.65Å resolution

crystal structure of SsGBP and GDP complex

Released:
DOI: 10.2210/pdb3kxi/pdb
PDB entry 3kxi coloured by chain, front view.
PDB entry 3kxi coloured by chain, side view.
PDB entry 3kxi coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family.
Huang B, Wu H, Hao N, Blombach F, van der Oost J, Li X, Zhang XC, Rao Z
J Biochem 148 103-13 (2010)
PMID: 20400571

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HflX Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 41.66 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980M3 (Residues: 1-356; Coverage: 100%)
Gene names: SSO0269, hflX
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand SCN 1 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 67.322Å b: 68.471Å c: 92.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.29
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The universally conserved prokaryotic GTPases.
Verstraeten et al. (2011)
6 other articles

1 mention without citation

Disassembly of the Staphylococcus aureus hibernating 100S ribosome by an evolutionarily conserved GTPase.
Basu et al. (2017)