› 3kwp

X-ray diffraction
2.29Å resolution

Crystal structure of putative methyltransferase from Lactobacillus brevis

Released:
DOI: 10.2210/pdb3kwp/pdb
PDB entry 3kwp coloured by chain, front view.
PDB entry 3kwp coloured by chain, side view.
PDB entry 3kwp coloured by chain, top view.
Source organism: Levilactobacillus brevis ATCC 367
Entry authors: Chang C, Xu X, Cui H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase I Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.88 KDa
Source organism: Levilactobacillus brevis ATCC 367
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03SR9 (Residues: 1-293; Coverage: 100%)
Gene names: LVIS_0608, rsmI
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 64.275Å b: 79.206Å c: 54.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.288
Expression system: Escherichia coli BL21(DE3)

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