› 3kvn

X-ray diffraction
2.5Å resolution

Crystal structure of the full-length autotransporter EstA from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3kvn/pdb
PDB entry 3kvn coloured by chain, front view.
PDB entry 3kvn coloured by chain, side view.
PDB entry 3kvn coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of a full-length autotransporter.
van den Berg B
J Mol Biol 396 627-33 (2010)
PMID: 20060837

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase EstA Chains: A, X
Molecule details ›
Chains: A, X
Length: 632 amino acids
Theoretical weight: 68.55 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: O33407 (Residues: 25-646; Coverage: 100%)
Gene names: PA5112, estA, papA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand C8E 6 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P4212
Unit cell:
a: 143.826Å b: 143.826Å c: 186.883Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.255
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
23 more

29 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
28 more