› 3kty

X-ray diffraction
2.3Å resolution

Crystal Structure of Probable Methyltransferase from Bordetella pertussis Tohama I

Released:
DOI: 10.2210/pdb3kty/pdb
PDB entry 3kty coloured by chain, front view.
PDB entry 3kty coloured by chain, side view.
PDB entry 3kty coloured by chain, top view.
Source organism: Bordetella pertussis
Entry authors: Kim Y, Tesar C, Keigher L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA/rRNA methyltransferase SpoU type domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 173 amino acids
Theoretical weight: 18.67 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7VXA3 (Residues: 1-170; Coverage: 66%)
Gene name: BP1901
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 42.48Å b: 69.514Å c: 174.802Å
α: 90° β: 95.16° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression system: Escherichia coli BL21

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