3krd

X-ray diffraction
2.5Å resolution

Crystal Structure of Mycobacterium Tuberculosis Proteasome in complex with Fellutamide B

Released:
Source organism: Mycobacterium tuberculosis
Primary publication:
Fellutamide B is a potent inhibitor of the Mycobacterium tuberculosis proteasome.
Arch. Biochem. Biophys. 501 214-20 (2010)
PMID: 20558127

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only 28-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasome subunit alpha Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Molecule details ›
Chains: 1, A, B, D, F, I, K, M, O, Q, S, U, W, Y
Length: 248 amino acids
Theoretical weight: 26.91 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHU1 (Residues: 1-248; Coverage: 100%)
Gene names: Rv2109c, prcA
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: 2, C, E, G, H, J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: 2, C, E, G, H, J, L, N, P, R, T, V, X, Z
Length: 240 amino acids
Theoretical weight: 25.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WHT9 (Residues: 58-291; Coverage: 80%)
Gene names: Rv2110c, prcB
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 170.186Å b: 118.099Å c: 194.347Å
α: 90° β: 112.62° γ: 90°
R-values:
R R work R free
0.208 0.208 0.229
Expression system: Escherichia coli