› 3krb

X-ray diffraction
1.75Å resolution

Structure of Aldose Reductase from Giardia Lamblia at 1.75A Resolution

Released:
DOI: 10.2210/pdb3krb/pdb
PDB entry 3krb coloured by chain, front view.
PDB entry 3krb coloured by chain, side view.
PDB entry 3krb coloured by chain, top view.
Source organism: Giardia lamblia ATCC 50803
Primary publication:
Structure of aldose reductase from Giardia lamblia.
Ferrell M, Abendroth J, Zhang Y, Sankaran B, Edwards TE, Staker BL, Van Voorhis WC, Stewart LJ, Myler PJ
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 1113-7 (2011)
PMID: 21904059

Function and Biology Details

Reaction catalysed: 
Alditol + NAD(P)(+) = aldose + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent oxidoreductase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.8 KDa
Source organism: Giardia lamblia ATCC 50803
Expression system: Escherichia coli
UniProt:
  • Canonical: A8BGX6 (Residues: 1-313; Coverage: 100%)
Gene names: GL50803_007260, GL50803_7260
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
2 bound ligands:
Ligand UNX 2 x UNX
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 196.77Å b: 66.09Å c: 56.29Å
α: 90° β: 92.26° γ: 90°
R-values:
R R work R free
0.145 0.144 0.173
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Aldose reductase from Schistosoma japonicum: crystallization and structure-based inhibitor screening for discovering antischistosomal lead compounds.
Liu et al. (2013)
1 more

4 mentions without citation

Essential Oils and Terpenic Compounds as Potential Hits for Drugs against Amitochondriate Protists.
Menezes et al. (2023)
3 more