3kq4

X-ray diffraction
3.3Å resolution

Structure of Intrinsic Factor-Cobalamin bound to its receptor Cubilin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Cobalamin binding intrinsic factor Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 393 amino acids
Theoretical weight: 42.82 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P27352 (Residues: 25-417; Coverage: 99%)
Gene names: CBLIF, GIF, IFMH
Sequence domains: Domain of unknown function (DUF4430)
Structure domains:
Cubilin Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 457 amino acids
Theoretical weight: 51.63 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: O60494 (Residues: 932-1388; Coverage: 13%)
Gene names: CUBN, IFCR
Sequence domains: CUB domain
Structure domains: Spermadhesin, CUB domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA, MAX II BEAMLINE I911-3
Spacegroup: C2221
Unit cell:
a: 117.684Å b: 204.179Å c: 410.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.242
Expression systems:
  • Komagataella pastoris
  • Cricetulus griseus