› 3km3

X-ray diffraction
2.1Å resolution

Crystal structure of eoxycytidine triphosphate deaminase from anaplasma phagocytophilum at 2.1A resolution

Released:
DOI: 10.2210/pdb3km3/pdb
PDB entry 3km3 coloured by chain, front view.
PDB entry 3km3 coloured by chain, side view.
PDB entry 3km3 coloured by chain, top view.
Source organism: Anaplasma phagocytophilum str. HZ
Primary publication:
SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth J, Gardberg AS, Robinson JI, Christensen JS, Staker BL, Myler PJ, Stewart LJ, Edwards TE
J Struct Funct Genomics 12 83-95 (2011)
PMID: 21359836

Function and Biology Details

Reaction catalysed: 
dCTP + H(2)O = dUTP + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173616 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dCTP deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 22.9 KDa
Source organism: Anaplasma phagocytophilum str. HZ
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2GLJ4 (Residues: 1-185; Coverage: 100%)
Gene names: APH_0130, dcd
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

1 bound ligand:
Ligand IOD 16 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: R3
Unit cell:
a: 84.66Å b: 84.66Å c: 140.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure, mechanism, and inhibition of histone deacetylases and related metalloenzymes.
Lombardi et al. (2011)
3 more

6 mentions without citation

SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth et al. (2011)
5 more