› 3kjo

X-ray diffraction
1.8Å resolution

Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)

Released:
DOI: 10.2210/pdb3kjo/pdb
PDB 3kjo coloured by chain and viewed from the front.
PDB 3kjo coloured by chain and viewed from the side.
PDB 3kjo coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
How telomeric protein POT1 avoids RNA to achieve specificity for single-stranded DNA.
Nandakumar J, Podell ER, Cech TR
Proc. Natl. Acad. Sci. U.S.A. 107 651-6 (2010)
PMID: 20080730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NUX5 (Residues: 1-299; Coverage: 47%)
Gene name: POT1
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
DNA/RNA (5'-D(*T)-R(P*U)-D(P*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 101.867Å b: 103.591Å c: 71.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.238
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

10 review citations

POT1-TPP1 telomere length regulation and disease.
Aramburu et al. (2020)
9 more

3 mentions without citation

Unraveling secrets of telomeres: one molecule at a time.
Lin et al. (2014)
2 more