› 3kij

X-ray diffraction
1.8Å resolution

Crystal structure of the human PDI-peroxidase

Released:
DOI: 10.2210/pdb3kij/pdb
PDB entry 3kij coloured by chain, front view.
PDB entry 3kij coloured by chain, side view.
PDB entry 3kij coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two endoplasmic reticulum PDI peroxidases increase the efficiency of the use of peroxide during disulfide bond formation.
Nguyen VD, Saaranen MJ, Karala AR, Lappi AK, Wang L, Raykhel IB, Alanen HI, Salo KE, Wang CC, Ruddock LW
J Mol Biol 406 503-15 (2011)
PMID: 21215271

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186242 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable glutathione peroxidase 8 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 180 amino acids
Theoretical weight: 21.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TED1 (Residues: 38-209; Coverage: 82%)
Gene names: GPX8, UNQ847/PRO1785
Sequence domains: Glutathione peroxidase
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2
Unit cell:
a: 90.43Å b: 122.3Å c: 70.18Å
α: 90° β: 117.9° γ: 90°
R-values:
R R work R free
0.211 0.209 0.248
Expression system: Escherichia coli

Quick links

Citations

74 review citations

Oxidative stress and diabetes: what can we learn about insulin resistance from antioxidant mutant mouse models?
Styskal et al. (2012)
73 more

1 mention without citation

Unique Structural Fold of LonBA Protease from Bacillus subtilis, a Member of a Newly Identified Subfamily of Lon Proteases.
Gustchina et al. (2022)