› 3kdm

X-ray diffraction
1.5Å resolution

Crystal Structure of Human Anti-steroid Fab 5F2 in Complex with Testosterone

Released:
DOI: 10.2210/pdb3kdm/pdb
PDB 3kdm coloured by chain and viewed from the front.
PDB 3kdm coloured by chain and viewed from the side.
PDB 3kdm coloured by chain and viewed from the top.
Source organism: Homo sapiens
Entry authors: Niemi MH, Rouvinen J

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 218 amino acids
Theoretical weight: 23.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 225 amino acids
Theoretical weight: 23.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand TES 2 x TES
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 66.75Å b: 67.08Å c: 67.06Å
α: 81.32° β: 69.3° γ: 69.27°
R-values:
R R work R free
0.181 0.18 0.208
Expression system: Escherichia coli

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Citations

1 review citation

In situ click chemistry: from small molecule discovery to synthetic antibodies.
Millward et al. (2013)
3 other articles

9 mentions without citation

The V1V2 Region of HIV-1 gp120 Forms a Five-Stranded Beta Barrel.
Pan et al. (2015)
8 more