› 3kch

X-ray diffraction
1.94Å resolution

Baranase crosslinked by glutaraldehyde

Released:
DOI: 10.2210/pdb3kch/pdb
PDB entry 3kch coloured by chain, front view.
PDB entry 3kch coloured by chain, side view.
PDB entry 3kch coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet.
Salem M, Mauguen Y, Prangé T
Acta Crystallogr Sect F Struct Biol Cryst Commun 66 225-8 (2010)
PMID: 20208148

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132804 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand PTD 3 x PTD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P32
Unit cell:
a: 58.97Å b: 58.97Å c: 82.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.148 0.146 0.185
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Cellulase Immobilization on Nanostructured Supports for Biomass Waste Processing.
Sulman et al. (2022)
15 other articles

2 mentions without citation

Revisiting glutaraldehyde cross-linking: the case of the Arg-Lys intermolecular doublet.
Salem et al. (2010)
1 more