PDBe 3kal

X-ray diffraction
1.9Å resolution

Structure of homoglutathione synthetase from Glycine max in closed conformation with homoglutathione, ADP, a sulfate ion, and three magnesium ions bound

Released:

Function and Biology Details

Reaction catalysed:
ATP + gamma-L-glutamyl-L-cysteine + beta-alanine = ADP + phosphate + gamma-L-glutamyl-L-cysteinyl-beta-alanine
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 55.73 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9M426 (Residues: 1-499; Coverage: 100%)
Gene name: hGS
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P32
Unit cell:
a: 115.7Å b: 115.7Å c: 101.76Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.197 0.25
Expression system: Escherichia coli