› 3k9s

X-ray diffraction
1.55Å resolution

Crystal structure of the peroxide-bound manganese superoxide dismutase.

Released:
DOI: 10.2210/pdb3k9s/pdb
PDB 3k9s coloured by chain and viewed from the front.
PDB 3k9s coloured by chain and viewed from the side.
PDB 3k9s coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of peroxide-soaked MnSOD crystals reveals side-on binding of peroxide to active-site manganese.
Porta J, Vahedi-Faridi A, Borgstahl GE
J. Mol. Biol. 399 377-84 (2010)
PMID: 20417642

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 205 amino acids
Theoretical weight: 23 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00448 (Residues: 2-206; Coverage: 100%)
Gene names: JW3879, b3908, sodA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand PEO 3 x PEO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 100.849Å b: 107.418Å c: 180.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.27
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Peroxomanganese complexes as an aid to understanding redox-active manganese enzymes.
Leto et al. (2014)
1 more

2 mentions without citation

A Review of the Catalytic Mechanism of Human Manganese Superoxide Dismutase.
Azadmanesh et al. (2018)
1 more