X-ray diffraction
2.51Å resolution

Crystal structure of E. coli lipopolysaccharide specific CMP-KDO synthetase

Source organism: Escherichia coli K-12
Entry authors: Heyes DJ, Levy CW, Lafite P, Scrutton NS, Leys D

Function and Biology Details

Reaction catalysed:
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-deoxy-manno-octulosonate cytidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 29.46 KDa
Source organism: Escherichia coli K-12
  • Canonical: P04951 (Residues: 1-248; Coverage: 100%)
Gene names: JW0901, b0918, kdsB
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 124.47Å b: 77.18Å c: 143.63Å
α: 90° β: 90.03° γ: 90°
R R work R free
0.201 0.199 0.234