X-ray diffraction
1.9Å resolution

Crystal structure of E. coli lipopolysaccharide specific CMP-KDO synthetase in complex with CTP and 2-deoxy-Kdo


Function and Biology Details

Reaction catalysed:
CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-deoxy-manno-octulosonate cytidylyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 264 amino acids
Theoretical weight: 29.46 KDa
Source organism: Escherichia coli
  • Canonical: P04951 (Residues: 1-248; Coverage: 100%)
Gene names: JW0901, b0918, kdsB
Sequence domains: Cytidylyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P31
Unit cell:
a: 94.77Å b: 94.77Å c: 153.14Å
α: 90° β: 90° γ: 120°
R R work R free
0.153 0.151 0.182