› 3k81

X-ray diffraction
3.4Å resolution

Structure of the central interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies

Released:
DOI: 10.2210/pdb3k81/pdb
PDB entry 3k81 coloured by chain, front view.
PDB entry 3k81 coloured by chain, side view.
PDB entry 3k81 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies.
Wu M, Park YJ, Pardon E, Turley S, Hayhurst A, Deng J, Steyaert J, Hol WG
J Struct Biol 174 124-36 (2011)
PMID: 20969962

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212226 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MP18 RNA editing complex protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 164 amino acids
Theoretical weight: 18.11 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38B90 (Residues: 1-164; Coverage: 100%)
Gene name: Tb10.70.2090
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins
Single strand antibody VHH domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.97 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 80.03Å b: 68.605Å c: 93.335Å
α: 90° β: 102.23° γ: 90°
R-values:
R R work R free
0.269 0.267 0.32
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Nanobody stabilization of G protein-coupled receptor conformational states.
Steyaert et al. (2011)
6 more

4 mentions without citation

Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies.
Wu et al. (2011)
3 more