PDBe 3k7j

X-ray diffraction
1.9Å resolution

Crystal structure of the D100E mutant of the Indian Hedgehog N-terminal signalling domain

Released:
Source organism: Homo sapiens
Entry authors: He Y-X, Kang Y, Zhang WJ, Yu J, Ma G, Zhou C-Z

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indian hedgehog protein N-product Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 21.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14623 (Residues: 28-202; Coverage: 46%)
Gene name: IHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 84.16Å b: 52.4Å c: 34.66Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.204 0.2 0.276
Expression system: Escherichia coli