› 3k6r

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF putative transferase PH0793 FROM PYROCOCCUS HORIKOSHII

Released:
DOI: 10.2210/pdb3k6r/pdb
PDB entry 3k6r coloured by chain, front view.
PDB entry 3k6r coloured by chain, side view.
PDB entry 3k6r coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Entry authors: Chang C, Skarina T, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + 4-demethylwyosine(37) in tRNA(Phe) = S-methyl-5'-thioadenosine + 7-((3S)-3-amino-3-carboxypropyl)-4-demethylwyosine(37) in tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 32.58 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O58523 (Residues: 1-278; Coverage: 100%)
Gene names: PH0793, taw2
Sequence domains: Met-10+ like-protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 51.308Å b: 54.231Å c: 58.613Å
α: 90° β: 100.02° γ: 90°
R-values:
R R work R free
0.213 0.211 0.256
Expression system: Escherichia coli BL21(DE3)

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