› 3k4l

X-ray diffraction
1.75Å resolution

Pyranose 2-oxidase F454N mutant in complex with 2FG

Released:
DOI: 10.2210/pdb3k4l/pdb
PDB entry 3k4l coloured by chain, front view.
PDB entry 3k4l coloured by chain, side view.
PDB entry 3k4l coloured by chain, top view.
Source organism: Trametes ochracea
Primary publication:
Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase.
Spadiut O, Tan TC, Pisanelli I, Haltrich D, Divne C
FEBS J 277 2892-909 (2010)
PMID: 20528921

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 623 amino acids
Theoretical weight: 69.38 KDa
Source organism: Trametes ochracea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7ZA32 (Residues: 1-623; Coverage: 100%)
Gene name: p2o
Sequence domains: GMC oxidoreductase
Structure domains: Phage tail proteins - 2 layer sandwich fold

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand SHG 2 x SHG
Ligand MES 3 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P43212
Unit cell:
a: 101.572Å b: 101.572Å c: 250.052Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.209
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

Hydrogen peroxide elimination from C4a-hydroperoxyflavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group.
Sucharitakul et al. (2011)
16 more

1 mention without citation

Experimental and Computational Studies on Structure and Energetic Properties of Halogen Derivatives of 2-Deoxy-D-Glucose.
Ziemniak et al. (2021)