› 3k4d

X-ray diffraction
2.39Å resolution

Crystal structure of E. coli beta-glucuronidase with the glucaro-d-lactam inhibitor bound

Released:
DOI: 10.2210/pdb3k4d/pdb
PDB entry 3k4d coloured by chain, front view.
PDB entry 3k4d coloured by chain, side view.
PDB entry 3k4d coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Alleviating cancer drug toxicity by inhibiting a bacterial enzyme.
Wallace BD, Wang H, Lane KT, Scott JE, Orans J, Koo JS, Venkatesh M, Jobin C, Yeh LA, Mani S, Redinbo MR
Science 330 831-5 (2010)
PMID: 21051639

Function and Biology Details

Reaction catalysed: 
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucuronidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 605 amino acids
Theoretical weight: 68.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P05804 (Residues: 1-603; Coverage: 100%)
Gene names: JW1609, b1617, gurA, gusA, uidA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EVA 2 x EVA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 167.653Å b: 76.971Å c: 125.468Å
α: 90° β: 124.98° γ: 90°
R-values:
R R work R free
0.206 0.205 0.237
Expression system: Escherichia coli

Quick links

Citations

238 review citations

The gut microbiota, bacterial metabolites and colorectal cancer.
Louis et al. (2014)
237 more

10 mentions without citation

A New Paradigm in the Relationship between Gut Microbiota and Breast Cancer: β-glucuronidase Enzyme Identified as Potential Therapeutic Target.
Fernández-Murga et al. (2023)
9 more