› 3k2q

X-ray diffraction
2.5Å resolution

Crystal structure of Pyrophosphate-dependent phosphofructokinase from Marinobacter aquaeolei, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET MqR88

Released:
DOI: 10.2210/pdb3k2q/pdb
PDB entry 3k2q coloured by chain, front view.
PDB entry 3k2q coloured by chain, side view.
PDB entry 3k2q coloured by chain, top view.
Source organism: Marinobacter nauticus VT8
Entry authors: Seetharaman J, Lew S, Wang D, Neely H, Janjua K, Cunningham K, Owens L, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 420 amino acids
Theoretical weight: 45.81 KDa
Source organism: Marinobacter nauticus VT8
Expression system: Escherichia coli
UniProt:
  • Canonical: A1U3A9 (Residues: 1-420; Coverage: 100%)
Gene names: Maqu_2402, pfp
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 151.526Å b: 100.752Å c: 101.584Å
α: 90° β: 110.34° γ: 90°
R-values:
R R work R free
0.234 0.234 0.26
Expression system: Escherichia coli

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