› 3k0s

X-ray diffraction
2.2Å resolution

Crystal structure of E.coli DNA mismatch repair protein MutS, D693N mutant, in complex with GT mismatched DNA

Released:
DOI: 10.2210/pdb3k0s/pdb
PDB entry 3k0s coloured by chain, front view.
PDB entry 3k0s coloured by chain, side view.
PDB entry 3k0s coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS.
Lebbink JH, Fish A, Reumer A, Natrajan G, Winterwerp HH, Sixma TK
J Biol Chem 285 13131-41 (2010)
PMID: 20167596

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116461 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 799 amino acids
Theoretical weight: 89.47 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23909 (Residues: 2-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP*CP*AP*CP*CP*AP*GP*TP*GP*TP*CP*AP*GP*CP*GP*TP*CP*CP*TP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP*GP*AP*C*AP*CP*T*GP*GP*TP*GP*CP*TP*TP*GP*GP*CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.28 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 89.604Å b: 90.791Å c: 261.191Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Mismatch repair in Gram-positive bacteria.
Lenhart et al. (2016)
2 more

5 mentions without citation

Magnesium coordination controls the molecular switch function of DNA mismatch repair protein MutS.
Lebbink et al. (2010)
4 more