3k02

X-ray diffraction
1.55Å resolution

Crystal structures of the GacH receptor of Streptomyces glaucescens GLA.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of Salmonella typhimurium.

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acarbose/maltose binding protein GacH Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 43.96 KDa
Source organism: Streptomyces glaucescens
Expression system: Escherichia coli
UniProt:
  • Canonical: B0B0V1 (Residues: 37-424; Coverage: 96%)
Gene names: SGLAU_00965, gacH
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 72.177Å b: 72.177Å c: 160.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.243
Expression system: Escherichia coli